ChromaLink Maleimide Biotin Reagent (MVS-B-1012-010)

The ChromaLink Biotin, maleimide version reacts with thiols groups on proteins. Soluble in DMF. Although widely used by researchers labeling antibodies and other molecules with biotin, the HABA:avidin method for estimating the extent of biotinylation has limitations. Because there are not many other methods available for estimating the extent of biotinylation, researchers using the HABA:avidin method have learned to live with some disadvantages. ChromaLink Biotin accurately determines the degree of biotin incorporation without any of these disadvantages.

Applications include:

• Biotinylate protein or peptides
• Quantitate the extent of biotin labeling
• Optimize biotin-avidin complexes

Highlights:

1. A chromophore that absorbs strongly at 354 nm when coupled to protein; the chromophore, also covalently attached to the protein, is used to estimate the extent of labeling.
2. A long polyethylene glycol (PEG3) spacer, which reduces the tendency for aggregation of the labeled protein and minimizes steric hindrances related to the binding of biotin to biotin-binding proteins in affinity or detection applications
3. A maleimide group, known to efficiently couple to thiols.